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. 2022 Sep 26;12(12):2111–2121. doi: 10.1002/2211-5463.13488

Fig. 4.

Fig. 4

Epigallocatechin gallate blocks the formation of the trans‐SNARE complex. (A) Diagram of the trans‐SNARE formation assay. (B) Reconstituted t‐ and v‐SNARE liposomes were incubated at 4 °C for the indicated periods in the presence or absence of 10 μm EGCG before 10‐fold excess amount of inhibitory V2CD was added. The liposomes were subsequently solubilized and the t‐SNAREs were precipitated. The presence of FL VAMP2 in the precipitates was probed by western blotting, which was used as an indicator for the trans‐SNARE complex.