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. 2022 Oct 20;12(12):2179–2190. doi: 10.1002/2211-5463.13496

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© 2022 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — Confirmation of mitochondrial inner membrane localization of TMEM160. (A) Mitochondria were isolated from HepG2 cells as described in the “Materials and methods” and subjected to the alkali extraction assay. Immunoblot analysis was performed [AP, alkali‐resistant pellet fraction; AS, alkali‐soluble supernatant fraction; ATP5A, mitochondrial ATP synthase subunit alpha; HSPA9, mitochondrial Hsp70; MT, isolated mitochondria; MTCO1, mitochondrially encoded cytochrome c oxidase I; TOMM22, translocase of outer mitochondrial membrane 22; UQCRC2, ubiquinol‐cytochrome c reductase core protein 2; VDAC1, voltage‐dependent anion channel 1]. (B) the mitochondria isolated from HepG2 cells were treated with digitonin at the indicated concentration, followed by centrifugation to obtain the supernatant and pellet fractions [P, pellet fraction; S, supernatant fraction].