Fig 3. Analysis of mismatch repair activity of the MLH1 variants.

DNA mismatch repair activity was assessed for wild-type and variant MLH1 proteins, and a negative control (without MLH1 protein) was included as detailed in “Materials and Methods”. A, Representative agarose gel image of the MMR activity measurement. The extent of repair is visible in the agarose gel electrophoresis by the generation of 2 smaller fragments (“Repair”) of the unrepaired, linearized plasmid (“No repair”). The shown agarose gel is cropped, a full view of the gel is provided in the supplementary data. B, 4 independent experiments were performed, and repair activity was scored relative to wild-type MLH1 protein (100%). Average repair values and standard deviations are shown.