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. 2022 Oct 24;7(20):e157502. doi: 10.1172/jci.insight.157502

Figure 4. PRC2 deletion upregulates expression of Mmp and Lox enzyme families.

Figure 4

(A) MMP enzyme gene expression by quantitative real-time PCR (qRT-PCR) in NP1, NP2, NC1, and NC2 isogenic cell lines shows upregulation of Mmp9 and Mmp2 with loss of either Eed or Suz12. Upregulation of Mmp14 is seen in some cells with PRC2 loss (n = 3). (B) Lox enzyme gene expression by qRT-PCR in NP1, NP2, NC1, and NC2 isogenic cell lines shows upregulation of Lox family genes LoxL2, LoxL3, and LoxL4 with loss of either Eed or Suz12 (n = 3). qRT-PCR data analyzed by 1-way ANOVA with Tukey’s multiple comparisons. Data represent biological replicates with the mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.