Skip to main content
. 2022 Oct 24;7(20):e157502. doi: 10.1172/jci.insight.157502

Figure 5. Inhibition of LOX enzyme function, but not MMP function, decreases PRC2-dependent metastatic phenotypes.

Figure 5

(A) Schematic of Transwell invasion across collagen with either nontargeting control siRNA (NT siRNA) or pooled Lox siRNA transfection (LoxL2/3/4 siRNA). (B and D) Representative images (original magnification, 20×) of cell Transwell invasion and clustering of NC1 (B) and NP1 (D) isogenic cell lines 48 hours posttransfection (n = 4). (C and E) Transwell invasion cell number (left) and cluster area (right) with either NT siRNA or LoxL2/3/4 siRNA 48 hours after transfection for NC1 (C) and NP1 (E) isogenic cell lines (n = 4). Transwell invasion assays show increased invasion through collagen of ΔEed and ΔSuz12 cells compared with nontargeting control by Kruskal-Wallis with multiple comparisons. Data represent biological replicates with the mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.