Skip to main content
. 2022 Oct 24;7(20):e144925. doi: 10.1172/jci.insight.144925

Figure 1. Canonical TGF-β signaling in the mouse distal hind limb where HO forms.

Figure 1

Immunofluorescence (IF) images, with (A) micro computed tomography (MicroCT) graphic showing the histology section level used at time points indicated. (B) Effects of TGF-β signaling by proxy of p-SMAD3 (green) and nuclear Hoechst (blue) in uninjured, 1 week after injury, and 3 weeks after injury. White chevrons point out cells as examples of positive p-SMAD3 staining. Scale bars represent 100 μm. (C) Quantification for percentage area of p-SMAD3 at uninjured (n = 3/group, 2–3 images/n mice) 1 week postinjury (n = 3/group, 3 images/n mice), 3 weeks postinjury (n = 3/group, 3 images/n mice). Error bars represent mean ± SEM.