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. 2022 Oct 24;7(20):e144925. doi: 10.1172/jci.insight.144925

Figure 8. Ligand trap does not change in MSC canonical signaling or proliferation but trends toward decreased Macs.

Figure 8

(A) IF tile scans of the distal hind limb where the tendons are outlined in white. The yellow box indicates the zoomed-in image location to the right of the tile scan where the 2 groups are identified above the images and the color legend is below. (B) Canonical TGF-β signaling by p-SMAD3 nuclear percentage in PDGFRα+ (MPCs) cells (n = 4/group, 3 images/n). (C–E) Further quantification from A, of the nuclei at injury site, total MPCs, and percentage of MPCs from total. (F) IF images and (G) quantification of proliferation by anti–Ki-67 in MPCs (n = 4/group, 3 images/n). (H) IF images and (I) quantification of percentage of Macs by anti-F4/80 (n = 4/group, 2–3 images/n). Error bars presented in graphs represent mean ± SEM for parametric data and represent median ± interquartile range for nonparametric data. Scale bars represent 100 μm.