(A) The pan-genome of 260 A. fumigatus strains included 15,508 gene families in total, including 8,595 (55.42%) core genes (present in >95% of strains), 3,660 (23.60%) accessory genes (present in >2 and <248 strains), and 3,253 (20.98%) singletons (present in only 1 isolate). (B) The distribution of the number of genomes represented in each gene family. (C) Gene family accumulation curves, including (green) and excluding (yellow) singletons. (D) The distribution of unique accessory gene families by strain. (E) The distribution of unique singleton gene families by strain. The data underlying this figure can be found in DOI: 10.5281/zenodo.5775265.