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. 2022 Nov 1;11:e80865. doi: 10.7554/eLife.80865

Figure 5. Lowering cholesterol levels aggravates hair cell defects in minar2 mutants.

(A) Quantification of D4H-mCherry intensity in wild type and mutant minar2fs139 larvae after 2HPβCD treatment. The lateral line L3 neuromast was imaged and quantified. n=45, 41, 52, and 51. F(3, 110.2)=74.76, p<0.0001. (B) Quantification of AM1-43 labeling in wild type and mutant minar2fs139 larvae after 2HPβCD treatment. n=49, 46, 49, and 51. F(3, 155.4)=124.4, p<0.0001. (C) Quantification of D4H-mCherry intensity in wild type and mutant minar2fs139 larvae after U18666A treatment. n=40, 31, 42, and 33. F(3, 111.3)=39.34, p<0.0001. (D) Quantification of AM1-43 labeling in wild type and mutant minar2fs139 larvae after U18666A treatment. n=53, 50, 52, and 46. F(3, 169.9)=158.0, p<0.0001. EM: embryonic medium, solvent control groups for 2HPβCD treatment (A and B); DMSO: solvent control groups for U18666A treatment (C and D); Multiple comparison significance values are indicated on the graph. Scale bars represent 10 μm.

Figure 5—source data 1. Effects of decreasing cholesterol levels on hair cells in minar2 mutant Figure 5A–D, Figure 5—figure supplement 1A–B.

Figure 5.

Figure 5—figure supplement 1. Effects of lowering cholesterol levels on hearing and neuromast hair cell numbers.

Figure 5—figure supplement 1.

(A) Effects of 2HPβCD treatment on C-start response rates for wild type and minar2fs139 mutants (n=48 for all 4 groups. p<0.0001, Kruskal-Wallis test). (B) Effects of 2HPβCD and U18666A treatment on the numbers of hair cells in lateral line L3 neuromast (for the 2HPβCD treatment, n=27, 32, 27, and 31, F(3,113) = 8.326, p<0.0001; for the U18666A treatment, n=30, 25, 28, and 27, F(3, 106)=28.22, p<0.0001. One-way ANOVA test.). EM: embryonic medium, solvent control groups for 2HPβCD treatment; DMSO: solvent control groups for U18666A treatment. Multiple comparison significance values are indicated on the graph.