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. 2022 Aug 26;55(12):e13325. doi: 10.1111/cpr.13325

FIGURE 2.

FIGURE 2

ZBTB11 promotes the proliferation and growth of BC cells. (A) The expression of ZBTB11 in T24 and UMUC3 cells with stable knockdown of ZBTB11 was detected using Western blot analysis. GAPDH was used as an internal control. (B) The proliferation of T24 and UMUC3 cells with stable knockdown of ZBTB11 was evaluated by MTT assay. The relative cell growth curves are presented. Statistical significance was calculated using two‐way analysis of variance. (C) The colony formation ability of stable knockdown BC cells was evaluated by colony formation assay. The numbers of colonies are presented as a bar chart. Statistical significance was calculated using one‐way analysis of variance. (D) Rescue of ZBTB11 expression was achieved by transfecting shRNA‐insensitive ZBTB11 vector into ZBTB11 knockdown BC cells. The expressions of ZBTB11 were detected by Western blot analysis. (E, F) The proliferation and colony formation ability of ZBTB11‐rescued ZBTB11 knockdown BC cells were evaluated by MTT assay and colony formation assay, respectively. The relative cell growth curves and numbers of colonies are shown. (G, H) The tumorigenic ability of UMUC3 cells with ZBTB11 knockdown was assessed by subcutaneous xenograft assay. Images of fixed tumours are presented. Tumour volumes at indicated days were recorded and graphed. Statistical significance was assessed by two‐way analysis of variance.