FIGURE 3.

Knockdown of ZBTB11 induces apoptosis in BC cells. (A) The expression of ZBTB11 in T24 cells was knocked down using CRISPR–Cas9 with three independent sgRNAs. The knockdown efficiency was evaluated by Western blot analysis. (B) GSEA plots of apoptotic pathway were analysed from the RNA‐Seq data of T24 cells with ZBTB11 knockdown. (C) The expression of proteins from the apoptotic pathway in ZBTB11 knockdown BC cells was detected by Western blot analysis. (D, E) The apoptosis of ZBTB11 knockdown BC cells was analysed by flow cytometry. The percentages of apoptotic cells are presented as a bar chart. Statistical significance was assessed using one‐way analysis of variance.