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Southern hybridization of genomic DNA from Tcr transconjugants with a Tn4400 probe. DNA samples were digested with AvaI. Lanes 1 and 15, HindIII-digested bacteriophage λ marker; lane 2, parent strain ATCC 33277; lanes 3 to 14, 12 randomly picked transconjugants. Solid arrowheads indicate the three internal AvaI-hybridizing bands of Tn4400′; open arrowheads represent the AvaI bands containing the end of IS4400L or IS4400R and the adjacent genomic fragment.
