Figure 5.

SlRIPK overexpression transgenic plants show increased ROS production after elicitor treatment. A Relative expression levels of SlRIPK in Micro-Tom (WT), 35S::GFP (GFP-OE), and 35S::SlRIPK-GFP (SlRIPK-OE) transgenic plants. RNA was extracted from 4-week-old leaves, and gene expression was quantified using qRT-PCR. Data are presented as the mean ± SE (n = 3). Different letters above the bars indicate significant differences between the different genotypes (P ≤ 0.05, one-way ANOVA). B, C Protein levels in GFP-OE and SlRIPK-OE transgenic lines. Total protein was extracted from 4-week-old leaves. Free GFP and SlRIPK-GFP were detected using an immunoblot analysis and an α-GFP antibody. Ponceau S staining of the membrane served as the loading control. D, E, FSlRIPK-OE transgenic plants showed increased ROS production after treatment with 20 μg/mL chitin (D), Pseudomonas syringae pv. tomato (Pst) DC3000 (avrRpm1) at an OD₆₀₀ of 0.3 (E), and 1 mM of Pip (F). Data are shown as the mean ± SE (n = 8). Different letters above the bars indicate significant differences between different genotypes (P ≤ 0.05, one-way ANOVA). The experiment was repeated three times, with similar results.