FIGURE 3.
Comparison of the FK506 production of the wild type and the overexpression strains with optimized lysine supply. Y-axis on right: percentage representation of production optimization; as 100% the FK506 production of the wild type (WT) was set at the respective time. Y-axis on left: calculation of the mean of specific FK506 production over a fermentation period of 6 days. pRM4 stays for the integrative plasmid without an inserted gene. pRM4/–integration of an additional copy of the gene on the integrative plasmid pRM4 encoding: askSt–aspartate kinase; dapASt - dihydropicolinate synthase; askSt*—feedback insensitive aspartate kinase; lysCCg*—deregulated aspartate kinase from C. glutamicum.