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. 2022 Nov 18;10:1067467. doi: 10.3389/fbioe.2022.1067467

FIGURE 8.

FIGURE 8

Engineering of PipAf. Key amino acid residues in the substrate-binding domain of PipAf are marked in red; the replacement of Ile91 by Val is marked in light green and labeled. The dimer is shown in green (first monomer) and yellow (second monomer). Amino acid residues are demonstrated on one dimer. Zoom-in represents the substrate binding domain (7-stranded β-barrel on each monomer).