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. 2022 Nov 18;13:1070940. doi: 10.3389/fmicb.2022.1070940

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Copyright © 2022 Lin, Zheng, Li, Wang, Wei, Xie, Lu, Tian, Wang, Qu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Integration of smartphone-controlled POCT with the closed-lid, CALIBURN-v2. (A) Schematic illustration of smartphone-controlled signal readout using the POCT device. The detection results are sent to the 5G cloud for subsequent analysis. The red dots denote the locations of positive cases in the map. (B) Sensitivity of CALIBURN-v2 on viral nucleic acid from clinical specimens. (C) Specificity of CALIBURN-v2. The RT-RPA primers and crRNAs for SARS-CoV-2 are used to detect nucleic acid samples from healthy donors, respectively. (D) Concordance between RT-qPCR and CALIBURN-v2 for SARS-CoV-2 detection. PPA, positive predictive agreement; NPA, negative predictive agreement; CI, confidence interval.