Skip to main content
. 2022 Dec 2;13:7424. doi: 10.1038/s41467-022-35234-0

Fig. 5. Comparison of AtSPY and other GT41 proteins.

Fig. 5

a Structural superposition of the active sites in the Arabidopsis ‘catalytic SPY’/GDP/‘substrate SPY’ complex and the human OGT/UDP-GlcNAc/HCF-11–26 complex (PDB code 4N3C) was performed using the catalytic domains as a reference. For Arabidopsis ‘catalytic SPY’/GDP/‘substrate SPY’ complex, GDP and some residues discussed in the text are shown as sticks. Specially, Y744 and S21* are also shown in dot representation. For simplicity, for the human OGT/UDP-GlcNAc/HCF-11–26 complex only the UDP-GlcNAc is shown as stick and colored in cyan. The figure is in wall-eyed stereo. b Structure-based sequence alignment of Arabidopsis SPY (AtSPY), Thermobaculum terrenum OGT (TtOGT; PDB 5DJS), Xanthomonas campestris OGT (XcOGT; PDB 2JLB), Actinobacillus pleuropneumoniae HMW1C protein (ApHMW1C; PDB 3Q3H), Drososphila melanogaster OGT (DmOGT; PDB 5A01) and human OGT (HsOGT; PDB 4N3C). Y744 in AtSPY is indicated using a green star. Only a short fragment containing Y744 in AtSPY is shown here. Sequence alignment of the connector regions and catalytic domains is shown in Supplementary Fig. 11.