Table 2.

Primers and probes used for qPCR assay with corresponding annealing/extension temperatures and amplified fragment length.

Organism	Target	Oligo ID	Sequence 5’-3’	Length (nt)	Fluorophore	Quencher	Ta (°C)	Fragment (nt)
Arsenophonus	16S rRNA	PRA-Ars1-F	TATCGCAGGAGAAAAGTCTG	20	–	–	60	91
Arsenophonus		PRA-Ars1-R	GCCCTACTCTTTGAGTTCAC	20	–	–
Arsenophonus		PRA-Ars1-P	ACCGGCAATAAAGGGTAATAGCCC	24	TAMRA	BHQ®-2
Portiera	16S rRNA	PR-Port1-F	GCAGAAGAGGAAGGTAGAA	19	–	–	60	110
Portiera		PR-Port1-R	TTCGCATCTCAGTGTCAG	18	–	–
Portiera		PR-Port1-P	CGCCTTCGCAACTGGTATTCC	21	HEX	BHQ®-1
Rickettsia	gltA	PR-Rick1-F	CTCCGCAAATGTTCACAG	18	–	–	60	102
Rickettsia		PR-Rick1-R	GTCTGCTGATTTTCTGCTC	19	–	–
Rickettsia		PR-Rick1-P	TCCATTGTGCCATCCAGCCTA	21	6-FAM	BHQ®-1
B. tabaci	Actin	SX-Actin1-F	CCCATCTACGAAGGTTAC	18	–	–	55	98
B. tabaci		SX-Actin1-R	CGTTCAGTGAGGATTTTC	18	–	–
B. tabaci		SX-Actin1-P	TCAAGTCACGACCAGCCAAGT	21	6-FAM	BHQ®-1
B. tabaci	COX1	SX-COX1-F	GGTGGTTTTGGTAATTGG	18	–	–	55	98
B. tabaci		SX-COX1-R	GAAGGAACTAAAAGTCAAAAC	21	–	–
B. tabaci		SX-COX1-P	CCTCTGATAATTGGTGCTCCTGACA	25	HEX	BHQ®-1