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. 2022 Dec 1;13:7408. doi: 10.1038/s41467-022-35069-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic of the experiment to determine the effect of Gpr151 loss on metabolic health in mice using the DIO model. Image created using Biorender. b Body weight in male DIO KO and WT mice over 12 weeks of HFD (N = 13, WT; N = 15, KO). Data are presented as mean values ±SEM. c Blood glucose levels measured during glucose tolerance testing in Gpr151 KO and WT DIO males. Data are presented as mean values ±SEM. Area of the curve (AOC) compared using two-tailed Student’s t test. Student’s t test with Bonferroni correction used to test differences at every time point (N = 9, WT; N = 9, KO; *t = 120 q_val = 0.02). d Blood glucose levels measured during insulin tolerance testing in Gpr151 KO and WT in DIO male mice (N = 10, WT; N = 9, KO). Data are presented as mean values ±SEM. AOC compared using two-tailed Student’s t test. e Fasting glucose levels in Gpr151 WT and KO DIO male mice measured in whole blood (N = 10, WT; N = 9, KO). Data are presented as mean values ±SEM. Two-tailed t Student test. f Fasting insulin levels measured in blood plasma of DIO male mice (N = 9, WT; N = 8, KO). Data are presented as mean values ±SEM. Two-tailed Student’s t tests. g–n Metabolic phenotyping of DIO male mice using CLAMS, conducted at 23 °C. g Representative time course of oxygen consumption (N = 5, WT; N = 5, KO). h Average oxygen consumption (N = 10, WT, N = 10, KO). Data are presented as mean values ±SEM. Two-tailed Student’s t tests. i Representative time course of CO₂ production (N = 5, WT; N = 5, KO). j Average CO₂ production (N = 10, WT; N = 10, KO). Data are presented as mean values ±SEM. Two-tailed Student’s t tests. k Average respiratory exchange ratio (N = 10, WT; N = 10, KO). Data are presented as mean values ±SEM. Two-tailed Student’s t tests. l Average locomotion measured as average number of beam breaks per hour (N = 9, WT; N = 8, KO). Data are presented as mean values ±SEM. Two-tailed Student’s t tests. m Representative time course of energy expenditure (N = 5, WT; N = 5, KO). n Average energy expenditure (N = 10, WT; N = 10, KO). Data are presented as mean values ±SEM. Two-tailed Student’s t tests. *p < 0.05. Source data are provided as a Source Data file.