Fig. 7. Cellular localization of the wild-type and p.Arg95Ter GPR151.

a Schematic of the overexpression plasmids used. EGFP and HA-tagged GPR151 are produced in equal ratio from a single transcript. Image created using Biorender. b Confocal images of EGFP-positive HEK293T cells that were transfected with each of the plasmids, fixed, permeabilized and fluorescently stained against the HA tag. Results of one experiment representative for three independent experiments. Nuclei counterstained with Hoechst. Images from a single z-plane, 60x objective. Scale bar: 10 μm. c Quantification of the ratio between total HA staining (AlexaFluor647) and total GFP signal over the entire cell area in a single confocal z-plane (n = 32, GPR151^WT; n = 17, GPR151^Arg95Ter). Average and S.E.M. are indicated. Two-tailed Student’s t test; ****p < 0.0001. Data are presented as mean values ±SEM. Source data are provided as a Source Data file.