Table 5.
SRY primers and Master Mix for the qPCR.
| SRY primers (5′—> 3′) | |
|---|---|
| Forward | CATGAACGCATTCATCGTGTGGTC |
| Reverse | CTGCGGGAAGCAAACTGCAATTCTT |
| Master Mix | 1X |
|---|---|
| Supermix 5X | 4 µl |
| Primer F | 0.4 µl |
| Primer R | 0.4 µl |
| ddH2O | 14.2 µl |
| DNA | 1 µl |
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