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. 2022 Dec 1;13:7419. doi: 10.1038/s41467-022-35094-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Spatial deconvolution result of pancreatic ductal adenocarcinoma (PDAC). b Three manually segmented annotation of PDAC tumor cryosection on one ST slide. The red line circles the ductal epithelium region (left), and the yellow line circles the cancer region (right). c Proportion and distribution of typical clusters in PDAC. d Expression of marker genes corresponding to clusters in c. e Distribution of cancer clone subtypes. f Top enriched KEGG terms of distinct cancer subtypes. g Boxplots of significant differences of cluster composition between the cancer clone A (n = 36) and cancer clone B (n = 41) regions (two-sided t-test). In the boxplots, the center line, box limits and whiskers denote the median, upper and lower quartiles and 1.5 × interquartile range, respectively.