Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 1;13:7402. doi: 10.1038/s41467-022-35030-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Protein melting point analysis of the stability-optimized RhlR-P75 vs. wildtype RhlR in the presence or absence of C4-HSL or mBTL. b Crystal structure of RhlR-P75 in complex with mBTL. Positions that have been altered with respect to the wildtype protein are shown in white. The magnified insert shows a cross-eyed stereo plot of mBTL in the ligand binding site. c Stability-optimized RhlR-P61 (RhlR-P75 with WT-DBD) stimulates pyocyanin production in a P. aeruginosa PA14 ΔrhlR/rhlI/pqsE triple mutant without requiring C4-HSL or mBTL. Mean values with error bars indicating the standard deviation of three independent measurements are shown. Source data are provided as a Source Data file.