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. 2022 Dec 1;12:20739. doi: 10.1038/s41598-022-24777-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Membership-based representation of unique, shared and core bacterial community of rumen microbiome after treatment supplementation with varying level of buffering capacity, and the total size of observed species per treatment. Venn diagram was generated in MetaCOMET²⁴ using jvenn²⁶. (b) Principal Coordinate Analysis (PCoA) of all samples using Bray–Curtis distance derived from the subset of identified OTUs. PCoA plot was generated using EMPeror²⁷. CON (no buffer added); BC_0.3% (0.3% buffer); BC_0.5% (0.5% buffer); BC_0.9% (0.9% buffer).