Figure 2.

Flow cytometric analyses of IPLuM and IPIM. IPLuM (A–C) or IPIM (D–F) were treated with (B,E) or without 1 μg/mL LPS (A,D) for 1 day. Then, the cells were reacted with mouse monoclonal anti-CD163 (blue line), anti-CD203a (green line), or anti-MHC-II (red line) antibodies, before being labeled with Alexa Fluor 488-conjugated anti-mouse IgG antibodies (Alexa 488). The cells treated with Alexa 488 alone were used as a negative control (NC) (black line). The flow cytometry histograms are representative of three independent experiments (A,B,D,E), and the MFI data are expressed as mean ± SEM values (n = 3, **p < 0.01 vs. NC) (C,F).