FIG. 2.

Nucleotide sequence of maa1 from M. arthritidis 158p10p9 (uppercase letters, first line), PG6, 158, and H39 (lowercase letters, second line). PCR primers PF4 and PR2, used for amplifying this region from LA, 158, and H39, are underlined and italicized. The sequence corresponding to the degenerate oligonucleotide probe is shaded in black. The probe was designed with an A residue instead of the authentic G (unshaded) at the sixth position. A putative ribosome binding site (RBS) and the translation start site are underlined and marked by arrows. A TAG stop codon is underlined (nt 2250), and a putative Rho-independent transcription terminator is boxed. A “-” indicates that the nucleotide sequence is the same as for 158p10p9; an asterisk indicates that nucleotides are missing from those sites in strains PG6, 158, and H39. Sites at which PG6, 158, or H39 differ from each other are annotated. A C→A substitution at nt 204 (underlined) generates premature TAA stop codons in strains 158 and H39. A G→T substitution at nt 695 (underlined) generates a premature TAA stop codon in maa1Δ. Otherwise, the sequence of maa1Δ from the LA variant is identical to that of maa1 from the 158p10p9 parent strain.