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. 2022 Nov 18;13:1044115. doi: 10.3389/fphar.2022.1044115

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Copyright © 2022 Ma, Tang, Tang, Wang, Zhang, Lu, Wu and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of A549 cell-derived CM on HUVEC angiogenesis. HUVECs were cultured with CM derived from A549 for 24 h, then a series of assays, including (A) tube formation (Scale bar, 100 μm), (B,C) wound healing (Scale bar, 200 μm), (D–G) Western blot, were performed to investigate effect of Curcumol on endothelial cells in tumor microenvironment. CM, conditioned medium. HUVEC, human umbilical vein endothelial cell. The data are presented from five independent experiments (n = 5) run in triplicate. *p < 0.05, compared with control (Con). #p < 0.05, compared with Con-CM.