FIGURE 4.

Overexpressed SP1 impacted Curcumol-mediated cell viability, VEGFA and miR-125b-5p. (A) A549 and H1975 were transfected with SP1 overexpression plasmid (pcDNA3.1-SP1 0.5 μg/ml) or control plasmid (pcDNA3.1) for 24 h, followed by added with Curcumol for another 24 h. Cell viability was calculated by OD (optical density) number of each well. (B,C) Protein expression level of SP1, VEGFA were detected by Western blot, with β-Tubulin as internal control. (D,E) The expression of VEGFA protein in A549 cells was detected by cellular immunofluorescence, and the localization and semi quantification were performed. DAPI was used to display nuclei. Scale bar, 20 μm. (F) NSCLC cells were transfected with vectors, followed by treating with Curcumol (final concentration 400 μM) for 24 h. Expression of miR-125b-5p were detected by qTR-PCR, with U6 as internal reference. The data are presented from five independent experiments (n = 5) run in triplicate. *p < 0.05, compared with control (Con). #p < 0.05, compared with pcDNA3.1 + Curcumol group.