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. 2022 Nov 18;9:1049910. doi: 10.3389/fvets.2022.1049910

Table 3.

Hatching performance after exposure to control or modified temperature during embryogenesis.

Items CT TMH TML P-value
BW (g) 40.64 ± 0.93 39.05 ± 1.10 42.99 ± 1.28 NS
YW (g) 4.53 ± 0.29 4.12 ± 0.27 5.15 ± 0.36 NS
YFBM (g) 36.11 ± 0.79 34.93 ± 0.87 37.65 ± 1.00 NS
Liver (g) 0.98 ± 0.03 0.95 ± 0.02 0.89 ± 0.03 NS
Heart (g) 0.29 ± 0.01 0.30 ± 0.01 0.31 ± 0.01 NS
Tona-score 90.6 ± 1.1 92.0 ± 1.5 91.4 ± 0.8 NS
Liver/BW (%) 2.39 ± 0.09a 2.45 ± 0.05a 2.06 ± 0.07b **
Heart/BW (%) 0.73 ± 0.03 0.77 ± 0.02 0.65 ± 0.05 NS
Liver/YFBM (%) 2.69 ± 0.11a 2.73 ± 0.05a 2.35 ± 0.07b **
Heart/YFBM (%) 0.82 ± 0.03 0.85 ± 0.26 0.75 ± 0.06 NS

The number of embryos used in each group was n = 8–10. Different superscripts in the same row indicate significant differences (P < 0.05) between treatments. Values are means ± SEM. CT, control treatment (37.6°C) during incubation; TMH, thermal manipulation with higher incubation temperature (39°C during ED 8–15 with daily 8-h treatment from 10:00 to 18:00) during incubation; TML, thermal manipulation with lower incubation temperature (20°C during ED 8–15 with daily 1-h treatment from 14:00 to 15:00) during incubation; ED, embryonic day; BW, body weight; YW, yolk weight; YFBM, yolk free body mass.

**

P < 0.01.

NS, not significant.