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. 2022 Nov 20;26(23):5917–5928. doi: 10.1111/jcmm.17615

FIGURE 2.

FIGURE 2

NP is a target of imR‐431‐5p and miR‐205‐5p. (A). Dual luciferase activity assay was performed by co‐transfected pmirGlo vector containing the NP gene sequence with miR‐205‐5p, miR‐431‐5p or miR‐744‐3p into 293 T cells. The dual luciferase activity assay was performed 24 h later. (B) Transfection efficiency of 293 T cells after 24 h (100×); (I) Light microscope of 293 T cells transfected with LV5 labelled negative control miRNA after 24 h, (II) Fluorescence microscope of 293 T cells transfected with LV5 labelled negative control miRNA after 24 h. n = 5, **p < 0.01. LV5, a fluorescent marker for labeling miRNA; NC, negative control miRNA