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Primer sequences in qPCR
| Gene | Primer type | Primer sequence |
|---|---|---|
| NP | Forward | GTGTATGGACCTGCCGTAGC |
| Reverse | TGTGCTGGATTCTCATTTGG | |
| GAPDH | Forward | AACGACCCCTTCATTGACCTC |
| Reverse | CCTTGACTGTGCCGTTGAACT | |
| miR‐431‐5p | Forward | ACGCTTGTCTTGCAGGCC |
| Reverse | TATGGTTCTTCACGACTGGTTCAC | |
| miR‐205‐5p | Forward | AAGCCGTTCCTTCATTCCAC |
| Reverse | TATGGTTGTTCTGCTCTCTGTCTC | |
| U6 | Forward | CGCTTCGGCAGCACATATAC |
| Reverse | TTCACGAATTTGCGTGTCATC |
