Figure 1.

A, Overview of mIHC staining and analysis pipeline including ROI selection from a PDAC resection, image registration, nuclei segmentation, color deconvolution, and single-cell measurements. B, Table describing biomarkers used for identifying cell types and functional states (left) alongside a visual of the gating strategy (right). The full gating strategy and description can be found in Supplementary Fig. S1. C, Gated phenotype map shows cells classified from manual gating (left); same region shown as a pseudofluorescent image (middle). White box indicates area of the right panel image; zoomed in region from middle panel image showing cells with biomarkers used for identification. White arrows (left to right) point to a CD3 T cell, CD20 B cell, CD8 T cell, and CD68 myelomonocytic cell. (Schematics created with BioRender.com.)