FIG. 2.

CD4⁺ T lymphocytes are required for optimal leukocyte accumulation in C. neoformans-infected brains of immune mice. Brains and spinal cords of immune mice depleted of CD4⁺ T cells contained significantly fewer leukocytes 6 days after i.c. infection with C. neoformans than those of CD8⁺ T-cell-depleted immune mice and immune mice with both CD4⁺ and CD8⁺ T cells. Leukocytes from five or six mice from each group were obtained as described in Materials and Methods and counted. Depletion of specific T-cell subsets by antibodies was confirmed by flow cytometric analysis of spleen cells from treated mice. The experiment was repeated once, with similar results.