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. 2022 Dec 2;12:20824. doi: 10.1038/s41598-022-25259-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Unsupervised analysis of the biological parameters considered: (a) response screen of the features as the function of the age showing the most discriminant factors. (b) PCA analysis of the best discriminators. (c) Hierarchical clustering of the best factors. Orange dots represent children, black adults, and pink young adults. Parameters in blue are downregulated and parameters in red are upregulated.