Table 1.

Flow cytometry panels used in the study.

	Panel	Commercial/ prototype	Conjugates	Format	Cytometer
Phenotyping	Infection related panel (surface)	Myeloid activation test + CD43	CD169-PE, HLA-DR-APC, CD64-PB, and CD43-AF750	Liquid	NAVIOS
	Granulocyte panel (surface)	DURAClone IM Granulocytes	CD294-FITC, CD16-ECD, CD33-PC5.5, CD11b-PC7, CD274-APC, CD3-CD19-CD56-CD14 APC-AF700, CD62L-APC-AF750, CD15-PB, and CD45-KrO	Dried	NAVIOS
	T cell subset panel (surface)	DURAClone IM T cell subset	CD45RA-FITC, CD197-PE, CD28-ECD, CD279-PC5.5, CD27-PC7, CD4-APC, CD8-AF700, CD3-APC-AF750, CD57-PB, and CD45-KrO	Dried	NAVIOS
	B cell subset panel (surface)	DURAClone IM B cells	IgD-FITC, CD21-PE, CD19-ECD, CD27-PC7, CD24-APC, CD38-APC-AF750, IgM-PB, and CD45-KrO	Dried	NAVIOS
	Dendritic cell panel (surface)	DURAClone IM Dendritic cells	CD16-FITC, CD3-CD14-CD19-CD20- CD56-PE, CD1c-PC5.5, CD11c-PC7, Clec 9A-APC, CD123-APC-AF700, HLA-DR-PB, and CD45-KrO;	Dried	NAVIOS
	Treg panel (intracellular)	DURAClone IM Treg	CD45RA-FITC, CD25-PE, CD39-PC5.5, CD4-PC7, FoxP3-A647, CD3-APC-AF750, Helios-PB, and CD45-KrO	Dried	NAVIOS
Phenotyping + functional analysis	Leukocyte activation panel (surface)	Prototype	CD54-FITC, CD11b-PE, CD16-ECD, CD56-PC5.5, CD69-PC7, CD62L-APC, tmTNF-AF700, CD3-CD66b-APC-AF750, CD14-PB, and CD45-KrO	Liquid	CytoFLEX
	Granulocyte activation panel (surface)	Prototype	DHR123-FITC**, MPO-PE, CD62L-ECD, CD11b-PC7, CD294-AF647, CD16-APC-AF700, CD66b-APC-AF750, DAPI-PB, and CD45-KrO	Liquid	CytoFLEX
	Cytokine activation panel (intracellular)	Prototype	IL8-A488*, IL6-PE*, CD14-ECD, IL4-PC7, CD3-APC, TNFa-A700, IFNγ-PB, and CD8-CD45-KrO	Liquid	NAVIOS
	Infection related panel (surface)	Myeloid activation test + CD43	CD169-PE, HLA-DR-APC, CD64-PB, and CD43-AF750	Liquid	NAVIOS

All the products are from Beckman Coulter except those indicated: *Product from Biolegend (San Diego, USA), **Product from Invitrogen (Waltham, USA).