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| Method | Vapour diffusion |
| Plate type | Sitting drop |
| Temperature (K) | 291 |
| Protein concentration (mg ml−1) | 25 |
| Buffer composition of protein solution | 50 mM HEPES pH 7.6, 100 mM NaCl |
| Composition of reservoir solution | 0.06 M magnesium chloride hexahydrate, 0.06 M calcium chloride dihydrate, 0.1 M Tris (base), 30%(v/v) Bicine pH 8.5, 40%(v/v) ethylene glycol, 20%(w/v) PEG 8000 |
| Volume and ratio of drop | 300 nl; 2:1 protein:reservoir solution |
| Volume of reservoir (µl) | 40 |
