| Method |
Vapor diffusion, sitting drop |
| Plate type |
24-well crystallization plate |
| Temperature (K) |
293 |
| Protein concentration (mg ml−1) |
20 |
| Buffer composition of protein solution |
20 mM potassium phosphate buffer pH 7.5, 0.1 mM EDTA |
| Composition of first reservoir solution |
100 mM Tris–HCl pH 8.5, 200 mM sodium acetate, 22%(w/v) PEG 4000 |
| Composition of second reservoir solution |
100 mM Tris–HCl pH 8.5, 200 mM sodium acetate, 16%(w/v) PEG 4000 |
| Volume and ratio of drop |
6 µl, 1:2 ratio of protein:reservoir solution |
| Volume of reservoir (µl) |
500 |
| Composition of the cryoprotectant |
20%(v/v) ethylene glycol in the second reservoir solution |
| Drop setting |
Manual |
| Seeding |
No |
