Table 1.

Sequence of primers for RT-PCR and long PCR.

Target Gene	Primer Sequence	Size (bp)	Tm (°C)
OPA1	Forward: 5’-TGGTTCGAGAGTCGGTTGAA-3’	189	56
	Reverse: 5’- CCTCCCAGTGCTTTGGAGTA -3’		56
Mfn1	Forward: 5’-GGGAAGACCAAATCGACAGA-3’	152	57
	Reverse: 5’-CAAAACAGACAGGCGACAAA-3’		57
Mfn2	Forward: 5’-GAGAGGCGATTTGAGGAGTG-3’	165	58
	Reverse: 5’-CTCTTCCCGCATTTCAAGAC-3’		56
Drp1	Forward: 5’-GCCCGTGGATGATAAAAGTG-3’	215	56
	Reverse: 5’-TGGCGGTCAAGATGTCAATA-3’		56
Fis1	Forward: 5’-AGATGGACTGGTAGGCATGG-3’	84	56
	Reverse: 5’-GACACAGCCAGTCCAATGAG-3’		56
PGC-1 α	Forward: 5’-GGACGAATACCGCAGAGAGT-3’	201	59
	Reverse: 5’-CCATCATCCCGCAGATTTAC-3’		56
Tfam	Forward: 5’-TCACCTCAAGGGAAATTGAAG-3’	241	55
	Reverse: 5’-CCCAATCCCAATGACAACTC-3’		56
Long Fragment	Forward:5’-AAAATCCCCGCAAACAATGACCACCC-3’	13400	72
	Reverse: 5’-GGCAATTAAGAGTGGGATGGAGCCAA-3’		72
Shrot Fragment	Forward: 5’-CCTCCCATTCATTATCGCCGCCCTGC-3’	235	60
	Reverse: 5’-GTCTGGGTCTCCTAGTAGGTCTGGGAA-3’		60
Bax	Forward: 5’-GCGATGAACTGGACAACAAC-3’	200	57
	Reverse: 5’-GATCAGCTCGGGCACTTTAG-3’		58
Bcl-2	Forward: 5’-CGAGTGGGATACTGGAGATGA-3’	236	58
	Reverse: 5’- GACGGTAGCGACGAGAGAAG-3’		59
Caspase-3	Forward: 5’-CCCATCACAATCTCACGGTAT-3’	195	57
	Reverse: 5’-GGACGGAAACAGAACGAACA-3’		58
Caspase-9	Forward: 5’-GCCTCTGCTTTGTCATGGAG-3’	181	56
	Reverse: 5’-AGCATGAGGTTCTCCAGCTT-3’		56
ASK1	Forward: 5’-ACAATGAGCAGACGATTGGC-3’	168	56
	Reverse: 5’-CAGCAAGCCTCTTGGATGTC-3’		56
JNK	Forward: 5’-TGGATTTGGAGGAGCGAACT-3’	69	56
	Reverse: 5’-TCACTGCTGCACCTAAAGGA-3’		56
Cyc-c	Forward: 5’-GGACAGCCCCGATTTAAGTA-3’	121	57
	Forward: 5’-TCAATAGGTTTGAGGCGACAC-3’		58
GAPDH	Forward: 5’- AGGTCGGTGTGAACGGATTTG -3’	20	58
	Reverse: 5’- GGGGTCGTTGATGGCAACA-3’		58