FIG. 3.

Phenotypes of CAF2 and the Δssk1 mutants (strains CSSK11-1, CSSK21-1, and CSSK23-1) grown under conditions of low nitrogen availability on solid SLAD medium. Plates were incubated for 8 days at 30°C. Invasion of the agar initially was observed after 5 days of incubation and increased with time. The colonies in the top row (UW, unwashed) show darker spots more clearly in the thinner areas of the colonies; these correspond to cells of the colonies that have invaded the agar. In the bottom row (W, washed), the same colonies are shown after the noninvading cells were washed off the surface of the agar. Bar, 1 mm.