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. 2022 Nov 30;10(11):e005517. doi: 10.1136/jitc-2022-005517

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© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Safety test on human RBCs in vitro. Hemagglutination of AK117 and Hu5F9-G4 (A). All versions of AK117 do not induce hemagglutination. Bivalent Hu5F9-G4 could induce hemagglutination but not monovalent version (Mo-Hu5F9). Phagocytosis of human RBCs by mouse bone marrow-derived macrophages mediated with AK117 and Hu5F9-G4 (B, n=6, one-way analysis of variance Newman-Kuels analysis, ***p<0.001, **p<0.01). FACS binding of AK117 and Hu5F9-G4 on human RBCs (C). PBS, phosphate-buffered saline; RBC, red blood cell; FACS, fluorescence activated cell sorting.