TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| GAS | ||
| MGAS 5005 | sic1.01; wild-type strain | J. Vuopio-Varkila |
| MGAS 5005 sic | sic-inactivated isogenic mutant strain | This study |
| MGAS 1600 | sic1.07; wild-type strain | K. Johnston |
| MGAS 1600 sic1 | sic::spc1; in-frame insertion | This study |
| MGAS 1600 sic2 | sic::spc2; out-of-frame insertion | This study |
| E. coli XL1-Blue | Cloning strain | Stratagene |
| Plasmids | ||
| pBC KS− | Cmr; E. coli cloning vector | Stratagene |
| pEU904 | Cmr Spr; source of aad gene | J. R. Scott |
| pSL60-1 to -3 | Cmr (Spr)a; spc1- to 3 cassettes in pBC KS− | This study |
| pFW12 | Spr; E. coli cloning vector | 27 |
| pFW14 | Cmr; source of cat gene | 27 |
| pLEX5B | Apr; ori ColE1; high-copy-number E. coli vector | 6 |
| pSL97 | Apr; oriR1; low-copy-number E. coli vector | This study |
| pSL99 | Apr; oriR1; sic1.07 | This study |
| pSL114 | Cmr; oriR1; sic1.07 | This study |
| pSL123 | Spr; 3′ flanking region of sic1.01 in pFW12 | This study |
| pSL124 | Spr; 5′ flanking region of sic1.01 in pSL123 | This study |
| pSL127 | Spr; sic1.01::spc2 in pSL124 | This study |
| pSL128-1 | Cmr; oriR1; sic1.07::spc1 | This study |
| pSL128-2 | Cmr; oriR1; sic1.07::spc2 | This study |
a
(Spr), spectinomycin resistance upon induction with IPTG.