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. 2022 Nov 30;10(11):e005619. doi: 10.1136/jitc-2022-005619

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© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Optical/X-ray imaging and contrast-enhanced ultrasound imaging follow-up of treated tumors among eight animal groups. The Optical/X-ray imaging (A) and contrast-enhanced ultrasound imaging (B) shows the tumors (golden-yellow color, red arrows) presented with smaller tumor size in the triple combination treatment (RFH+LTX-315 (L-315)+DOX) group, compared with other seven treatment groups (***p<0.001). (C, D) The quantitative analysis shows a significantly lower relative optical signal intensity and smaller relative tumor volume in the triple combination treatment (RFH+LTX-315 (L-315)+DOX) group, compared with other seven treatment groups (***p<0.001). (E) Tumor growth chart shows the tumors in the triple combination treatment group with the smallest tumor size at 7 and 14 days after treatments, compared with other seven treatment groups. n=6 in each group. Error bars represent SD. RFH, radiofrequency hyperthermia; SI, signal intensity.