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. 2022 Nov 30;8(2):e002672. doi: 10.1136/rmdopen-2022-002672

Figure 5.

Figure 5

LINC01871 is modulated by IFNγ and calcineurin/NFAT signalling in purified human T cells. (A–E) Negatively selected human donor T cells were treated with DMSO or 2.5 µM FK506 overnight, then stimulated with PMA/I (100 ng/mL; 1000 ng/mL) for 12 hours. Expression of LINC01871 (A), IL2 (B), IFNG (C), CD8A (D) and TBX21 (E) was assessed by RT-qPCR and reported as fold change relative to DMSO-treated cells; n=4; paired t-test where *p<0.05, **p<0.01, ***p<0.001 or ****p<0.0001. Grey lines indicate PMA/I. Black lines indicate PMA/I with FK506 treatment. (F–I) Negatively selected human donor T cells were treated with either DMSO or 2.5 µM FK506 overnight, then stimulated with anti-CD3/CD28 for 48 hours. Expression of LINC01871 (F), IL2 (G), IFNG (H) and CSF1 (I) was assessed by RT-qPCR and reported as fold change relative to DMSO-treated cells at each time point; n=4; paired t-test where *p<0.05, **p<0.01, ***p<0.001 or ****p<0.0001. Grey lines indicate anti-CD3/CD28. Black lines indicate anti-CD3/CD28 with FK506 treatment. IFN, interferon; PMA/I, phorbol 12-myristate 13-acetate and ionomycin; RT-qPCR, quantitative reverse transcription PCR.