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. 2022 Dec 2;22:517. doi: 10.1186/s12872-022-02959-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Circ_0004104 knockdown boosted proliferation and inhibited inflammatory in ox-LDL-exposed HUVECs. A Circ_0004104 level was determined in blood samples from 21 atherosclerosis patients and 17 healthy volunteers using qRT-PCR. B Circ_0004104 expression was measured in HUVECs treated with 0 μg/mL, 25 μg/mL, 50 μg/mL and 100 μg/mL of ox-LDL using qRT-PCR. C qRT-PCR analysis of circ_0004104 expression in HUVECs treated with or without RNase R assay. D qRT-PCR analysis of circ_0004104 expression HUVECs transfected with si-NC or si-circ_0004104. (E–J) HUVECs were treated with Control, ox-LDL, ox-LDL + si-NC, or ox-LDL + si-circ_0004104. E qRT-PCR analysis of circ_0004104 expression in treated HUVECs. F Cell proliferation was assessed in treated HUVECs using MTT assay. G Apoptosis rate was analyzed in treated HUVECs using flow cytometry assay. H The ability of HUVEC tube formation was assessed using tube formation assay. I and J Protein levels of CyclinD1 and Cleaved-caspase-3 in treated HUVECs were detected using western blot assay. K The secretion of inflammatory factors IL-1β and TNF-α in treated HUVECs was analyzed using ELISAs. **P < 0.01; ***P < 0.001; ****P < 0.0001. All cellular experiments were independently repeated three times. Student’s t-test was used to analyze the differences in (A and D), one-way ANOVA was utilized to assess the differences in (F, I, and K), and two-way ANOVA was utilized to analyze the differences in (B, C, G, and H)