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. 2022 Dec 1;17:37. doi: 10.1186/s13062-022-00344-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Synj2bp silencing reduces the extent, but not the distance, of the wrappER-mitochondria contact. A Schematic representation of the protocol used in this study. B Immunoblot and densitometry analysis showing Synj2bp silencing in the livers of mice injected with AAV8-shSynj2bp. C Comparative quantitative proteomic analysis. The volcano plot shows the proteins that are down-and up-regulated in WAM-enriched fractions isolated from Synj2bp-silenced livers compared to control AAV8-shScrambled injected mice. D, E Electron microscopy morphometric analyses of the wrappER-mitochondria contact distance (D) and the percentage of mitochondria perimeter covered by the wrappER (E) in control and Synj2bp-silenced mouse livers. Note that MAMs (magenta) were excluded from these analyses. The Student’s t test was used to calculate p values. Data were obtained from mouse livers at 3 h postprandial unless otherwise indicated. WAM: wrappER-associated mitochondria