Table 2.
Parameters required for the proposed HPLC method's system suitability testing
| Parameters | PAP | PCM | PHA | ETO | Reference value (50) |
|---|---|---|---|---|---|
| Resolution (Rs)a | 12.290 | 14.498 | 20.296 | Rs > 2 | |
| Selectivity factor (α)b | 2.372 | 1.545 | 1.504 | α > 1 | |
| Capacity factor (K) | 2.456 | 5.827 | 7.005 | 8.549 | 1 < K < 10 |
| Number of theoretical plates (N) | 2437 | 9838 | 19595 | 41923 | N > 2000 |
| Tailing factor (T) | 1.550 | 1.352 | 1.352 | 1.280 | T ≤ 2 |
a Resolution (Rs) = 2(tRB—tRA) / (WB+WA), where tR is the retention time and w is the peak width calculated for each of the two successive peaks
b Selectivity (α) = k’2/k’1 calculated for each of two successive peaks