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Stopped flow, pre-steady state fluorescent trace of (OH-)anthranilate (first product of the reaction) formation with: a, 25μM HsKYNase with 800 μM KYN. b, 5 μM HsKYNase_66 with 500 μM KYN. c, 5 μM HsKYNase with 500 μM DL-3’-OH-KYN. d, 12.5 μM HsKYNase_66 with 1000 μM DL-3’-OH-KYN. Each trace represents the average of five different experiments. Red traces: data points; black line: fit to 1-exponential burst equation (Equation 2 in Methods).
