. Author manuscript; available in PMC: 2023 Apr 19.

Published in final edited form as: Nat Catal. 2022 Oct 19;5(10):952–967. doi: 10.1038/s41929-022-00856-6

Table 1:

HsKYNase Variants of Interest

							% Stability in serum
Enzyme	# Mutations	List of Amino Acid Mutations	k_cat(s⁻¹)	K_M(mM)	k_cat/K_M (M⁻¹s⁻¹)	Fold HsKYNase	After 3 hours	After 24 hours
HsKYNase	0	N/A	0.18	1.57	110	1	17	2.7
HsKYNase_46	9	N67D/L72N/E103Q/M189I/F225Y/S274G/I331V/I405L/S408N	0.58	0.19	3100	28	0	0
HsKYNase_64	18	N67D/L72N/A99I/H102W/E103F/V104E/K106E/R107S/I110M/A136T/M189I/F225Y/S274G/I331C/N333T/S341I/I405L/S408N	0.24	0.036	6700	61	0	0
HsKYNase_66	24	N67D/L72N/A99I/H102W/E103F/V104E/K106D/R107S/T111H/G112Y/A132V/A136T/M189I/V223I/F225Y/S274G/A280S/G281S/A282P/I331C/N333T/S341I/I405L/S408N	1.54	0.027	56000	510	22	0
HsKYNase_95	25	N67D/A68T/L72N/A99I/H102W/E103F/V104E/K106D/R107S/I110L/T111H/G112Y/A136T/M189I/F225Y/S274N/A280S/G281S/A282P/F306W/I331C/N333T/S341I/I405L/S408N	0.85	0.016	55000	500	44	1.8