Figure 8.

Heme-mediated mitochondrial biogenesis and fission in human RPTECs. (A) Hemin treatment (25 µM, 8 hours) markedly decreased PGC-1α mRNA expression in human RPTECs as assessed by real-time RT-PCR analysis compared with vehicle treatment. Conversely, hemin treatment significantly increased p-DRP1 protein expression (B) with no change in overall DRP1 protein expression in human RPTECs (C) compared with vehicle treatment. Densitometry (mean±SEM), normalized for GAPDH expression, is displayed below blots. n=4 in each group. ***P<0.001 versus vehicle alone.