Skip to main content
. Author manuscript; available in PMC: 2022 Dec 2.
Published in final edited form as: Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2020 Jul 3;12(6):e1653. doi: 10.1002/wnan.1653

FIGURE 5.

FIGURE 5

a) Synthesis of Cas9 RNP-encapsulating NCs (i.e. CPNCs). b) Optimizing the NC formulation in vitro using mCherry-expressing HEK 293 (mCherry-HEK 293) cells. c) Gene editing in mCherry-HEK 293 cells with RNP-encapsulating NCs, as compared to unencapsulated RNP, optimized Lipofectamine (Lipo) system and untransfected control sample (UT). d) Deep sequencing of HEK 293 cells to examine in vitro gene editing efficiency with RNP-encapsulating NCs with and without decoration using cell-penetrating peptide (CPP). e) In vivo gene editing efficiency expressed as the percentage area of whole muscle tissue showing confocal fluorescent signal for a genome editing reporter (tdTomato+) 12 days after the intramuscular injection of genome editors. (Adapted with permission from Chen et al., 2019).